Treatment with DNA demethylating agents or DNMT/Dnmt inhibitors such as decitabine (also called 5-Aza-2′-deoxycytidine) is a commonly used method to study the role of DNA methylation in gene expression [29,30]. While exposure to decitabine results in DNA demethylation, its subsequent withdrawal causes remethylation or methylation reprogramming [29], providing an excellent platform to uncover the role of DNA methylation in gene expression.
