The expression and activation of OPC AMPARs is considered crucial to OPC physiology and has been implicated in oligodendrocyte pathologies 32, 33, 34. We further explored the AMPAR properties in hPSC‐derived oligodendroglial cells. Quantification of AMPAR subunit mRNA expression by quantitative real‐time PCR (qRT‐PCR) revealed that there was an increase in the GluA2, GluA3, and GluA4 mRNA expression levels relative to GluA1 in week 3 O4+‐oligodendrocytes compared with the levels observed in OPCs cultures in which OL differentiation was inhibited by absence of mitogen withdrawal (Fig. 6D). Our data indicate that GluA2 is the most prominently expressed AMPAR subunit in week 3 O4+‐oligodendrocytes.
