Chunk #7 — Material and Methods — Voluntary ethanol consumption using two-bottle choice paradigms — Ethanol intake procedures

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Varenicline, a partial agonist at neuronal nicotinic acetylcholine receptors, reduces nicotine-induced increases in 20% ethanol operant self-administration in Sprague-Dawley rats.

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All fluids were presented in 100-ml graduated glass cylinders with stainless-steel drinking spouts inserted through two grommets in front of the cage 15 min after the lights went off in the reversed light/dark cycle room. Bottles were weighed 30 min and 24 hours after the fluids were presented, and measurements were taken to the nearest gram. The weight of each rat was measured daily Monday through Friday to monitor health and calculate the grams of ethanol intake per kilogram of body weight.