AP: −6.72 mm from bregma; ML: ±1.6 mm from midline; DV: −6.5 mm from brain surface) or VTA (bilateral; flat skull; 6° angle toward midline; AP: −5.4 mm from bregma; ML: ±1.3 mm from midline; DV: −7.0 mm from skull)3. The MHb guide cannula coordinates were the same as for the lentiviral injections, except with DV at −2.9 mm from brain surface. For all of the cannula, injector needles extended 2 mm below the tip of the cannula for placement into the brain region. For the ICSS electrode, a stainless steel bipolar electrode (Plastics One) was implanted into the lateral hypothalamus (AP: −0.5 mm from bregma; ML: ±1.7 mm from midline; DV: −8.3 mm from brain surface)3.
