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Chunk #14 — Materials and methods — Brain tissue preservation

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The neuropathology of autism: defects of neurogenesis and neuronal migration, and dysplastic changes.
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The brain hemispheres were removed using standard techniques, exercising extra care to avoid damaging the brain tissue. The brain was weighed in the fresh state. The fresh brain was sagittally cut through the corpus callosum and brainstem. Half of the brain was fixed in 10% buffered formalin. Following at least 3 weeks of fixation, the brain hemisphere was scanned using MRI. The aim of the MRI application was to determine the type of developmental changes detectable by MRI and to microscopically characterize MRI findings. All brains within this project were scanned (L.A.S.L.) using a standardized protocol (established and implemented for this and for other postmortem MRI studies by L.A.S.L. and M.L.). MRI scans were acquired on a 1.5 T GE Signa Imager (General Electric, Milwaukee, USA). The research scan consisted of a 124-slice T1-weighted fast gradient echo acquired in a coronal orientation perpendicular to the long axis of the hippocampus with a 1.5-mm slice thickness, which encompassed the entire brain hemisphere without gaps or wrap artifacts (FOV = 25 cm; NEX = 1; matrix = 256 × 192; TR =