temperature in the dark for 30 minutes. This step was repeated and then 180 μl 0.02% Tween-20 was added. Beads were pelleted and washed in 0.1% SDS in TE, pH 8.0 buffer. Beads were stored in TE in the dark at 4°C for up to one month. Mixtures of beads were freshly prepared in 1.5× TMAC buffer (4.5 mol/l tetramethylammonium chloride, 0.15% N-lauryl sarcosine, 75 mmol/l tris-HCl [pH 8.0], and 6 mmol/l EDTA [pH 8.0]).
