The experiment was divided into 3 phases (see Figure 1): first-order conditioning, second-order conditioning and locational preference assessment. On P32 or P70 (first-order conditioning phase) animals were given intraoral sucrose infusion (CS1) either 5-20 or 30-45 min after ethanol administration. Ethanol-sucrose pairings were followed 24 hrs later by a brief exposure to the CS1 while subjects were placed in a distinctive chamber lined with sandpaper (CS2, second order conditioning phase). Thirty min later, subjects were evaluated in a 12 min place preference assessment (P33 or 71). A detailed account of conditioning and testing procedures follows:
