RNA extraction was performed using Trizol (Invitrogen) and was followed by Dnase I treatment, and cDNA conversion was performed using oligo(dT) primers (Invitrogen) according to manufacturers instructions (Roche Molecular Biochemicals). The same subjects used for methylation analysis were studied for expression analyses using quantitative RT-PCR. Outliers with expression values that differed more than 1.5 s.d. from the mean were excluded from analysis (n = 2 control subjects, n = 1 suicide victims with a history of childhood abuse for glucocorticoid receptor 1F and n = 2 control subjects, n = 2 suicide victims with a history of childhood abuse and n = 3 nonabused suicide victims for overall levels of glucocorticoid receptor; see Supplementary Methods online).
