Chunk #18 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.2. Lentiviral transduction — Day-1: Transduction

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Note: Third-generation VSV.G pseudotyped HIV-1 lentiviruses were produced by polyethylenimine (PEI, Polysciences #23966-2)-transfection of HEK293T cells and packaged with VSVG-coats using established methods, see [35].