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Chunk #11 — 2. Materials and methods — 2.4. Preference for non-ethanol tastants in two-bottle choice test

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Activation of inflammatory signaling by lipopolysaccharide produces a prolonged increase of voluntary alcohol intake in mice.
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Separate groups of B6 mice were also tested for saccharin, quinine, and sucrose consumption after treatment with LPS. Different groups of mice were serially offered sucrose (0.1%, 0.5%, 1.0%, 2.5%, and 5.0%), quinine hemisulfate (0.015 mM, 0.03 mM, and 0.06 mM) and saccharin (0.00165%, 0.033%, 0.066%, 0.132%, and 0.264%), and intakes for 24 h of drinking were calculated. Each concentration was offered for 2 d, with bottle positions changed every day. Within each tastant, the low concentration was always presented first, followed by the higher concentrations in increasing order.