processed for Western Blot analysis. The following antibodies were used: anti-GST (1:5000, Santa Cruz), anti-6×His (1:1000, Cell Signaling), anti-KIAA1212 (1:50, Immuno-Biological Laboratories, Inc.), anti-phospho-KIAA1212 (1:50, Immuno-Biological Laboratories, Inc), anti-DISC1 (1:1000, Santa Cruz), anti-phospho-AKT at Ser473 (1:1000, Cell Signaling), anti-AKT (1:1000, Cell Signaling), anti-phospho-S6 ribosomal protein (1:1000, Cell Signaling), anti-S6 ribosomal protein (1:1000, Cell Signaling), anti-PTEN (1:1000, Cell Signaling), anti-GAPDH (1:5000, AbCam). For quantification, the densitometry measurement of bands for phosphorylated proteins (Image J) was first normalized to that of total protein and then averaged from at least three independent experiments.
