tissues were collected: white quadriceps muscle (WQ), red quadriceps (RQ), red gastrocnemius (RG), epididymal adipose tissue (white fat), interscapular brown adipose tissue (brown fat), diaphragm, heart, and cerebellum. For measurement of total 3H-label content, freshly collected tissue pieces were weighed and placed in small cardboard cones for combustion (see below). Two blood samples (150 μL each) were collected; plasma was separated and stored at −20°C for later determination of steady state plasma glucose, insulin, and FFA concentrations: one was collected just prior to the tracer bolus, the other 45 min after the tracer bolus.
