Chunk #29 — MATERIALS — Procedure — PiggyBac transposon removal

Source

Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.

Embedded

yes

Text

CRITICAL: If multiple genome editing steps are anticipated, it may be desirable to leave the piggyBac transposon in place, and then remove it at a final independent step.