1% sucrose solution compared with AAV-GFP injected control mice (Fig. 4d, P = 0.012). Additionally, the latency to eat (P = 0.024) was increased, while food consumption during the 30-min period (P = 0.001) was significantly increased of AAV-Cre injected mice in the NSFT compared to mice injected with the AAV-GFP virus (Fig. 4e, genotype: F (1, 14) = 9.122, P = 0.009; timepoints: F (2, 28) = 235.300, P < 0.0001; genotype × timepoints: F (2, 28) = 6.132, P = 0.006). Finally, there were no differences in locomotor activity of AAV-Cre and AAV-GFP injected mice (Fig. 4f, distance travelled: genotype: F (1, 14) = 0.073, P = 0.792; timepoints: F (14, 196) = 23.500, P < 0.001; genotype × timepoints: F (14, 196) = 1.272, P = 0.851; total distance: P = 0.734).
