Chunk #11 — MATERIALS AND METHODS — Li treatment

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Neurons derived from patients with bipolar disorder divide into intrinsically different sub-populations of neurons, predicting the patients' responsiveness to lithium.

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Cultures were treated chronically with 1 mM LiCl starting at 14 days differentiation. Every second day, 50% of the differentiation medium containing 1 mM LiCl was exchanged. Electrophysiological recordings were conducted at 22–30 days differentiation.