Chunk #14 — METHODS — SVZ micro-dissection

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Notch and EGFR pathway interaction regulates neural stem cell number and self-renewal.

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SVZ areas were micro-dissected from 300μm-thick brain coronal sections. Single cell were dissociated19,38 on coated coverslips in 24-well plates (BDFalcon, Franklin Lakes, NJ). Coverslips were processed for immunocytochemistry 2h after plating.