1E) (Yu et al., 2012). It revealed that the modules formed distinct ontological clusters with little to no overlap. We also identified unique pathways for each module using the Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway (Fig. 1F) (Kanehisa et al., 2012). These pathways include gene regulation, metabolic pathways, neuronal activity and axon guidance as well as pathways involved with epigenetic regulation. Notably, we found that the turquoise module showed most significant association with methyltransferase activity and the light-yellow module showed most significant association with RNA polymerase II activity. This suggests that EtOH can potentially affect transcriptional activity and regulation in hESCs.
