Chunk #12 — Results — The Efficacy of dCas9-Mediated Transcript Activation Varies Extensively between Genes and Is Not Necessarily Consistent between Unique Donors
Altogether, these results demonstrate that designing three gRNAs is not necessarily sufficient to ensure successful manipulation of gene expression. Moreover, results are not always consistent across hiPSC-NPC lines derived from unique individuals. This variation could not be explained by differences in the levels of dCas9-effector protein (Figure S2A) or transcript (Figures S2B and S2C) between individuals. Therefore, we recommend validating gRNA efficacy across each individual hiPSC line to be evaluated.
