and provide documentation. MBF_ImageJ was developed by Collins and colleagues to provide a comprehensive user manual with an organized preloaded plugin and macro structure for ImageJ so that users could follow the instructions to do certain steps such as thresholding and 3D rendering. ImageJA was developed to allow for an applet version of ImageJ that could be run in any web browser and this is now integrated into Fiji. SalsaJ was a targeted version of ImageJ with an interface and content for astronomy users. There have also been several attempts to extend the functionality and data model of ImageJ including ImageJX and ImageJ2X. These are no longer active initiatives, but ideas from those projects have been incorporated in current ImageJ efforts including the ImageJ2 project. Other applications are not variants of ImageJ but use components of ImageJ such as the plugins; these include MicroManager10, Icy11, Cell Profiler5, 12, 13 and Bio7.