identify synapsin-positive puncta co-localized within a defined region of MAP2 signal (1.5 μm away from dendrite), used as the metric for synapse number. The quantification reproduced a trend consistent with previous literature, showing an increase in puncta density with NL3 overexpression (Fig. 4). This experiment demonstrates the utility of the 96 well device in performing morphological assays within the IN Cell Analyzer 6000 for high-throughput investigation of specific neuropathological mechanisms.
