Since the development of the methods that make generating stem cells from adult somatic cells possible, the field of stem cell research has been fueled with the potential that these methods can offer. Of particular interest, stem cell therapies have shown promise in treating neurological disorders. However, the differentiation stages of the NPCs that are generated from iPSCs are not described in detail. In the present study, we analyzed the differentiation processes of hiPSC-derived NPCs using multiple analyses. We first assessed the morphological changes and then examined the physiological function of hiPSC-derived NPCs during cell maturation. Lastly, we examined the whole cell gene expression pattern.
