To compare between different flux assay runs, the fluorescence was either normalized to the baseline fluorescence before CCCP (F b) and the fluorescence following Valinomycin (F v), or in the case of acutely adding a compound, the fluorescence after CCCP (F b) and the fluorescence following Valinomycin (Fv). This normalized fluorescence (F N), defined as ‘relative K+ flux”, was calculated according to the equation (Eq. 1):1\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${F}_{N}(t)=\frac{F(t)-{F}_{v}}{{F}_{b}-{F}_{v}}$$\end{document}FN(t)=F(t)−FvFb−Fvwhere F N (t) is the normalized fluorescence at time t and F(t) is the relative fluorescence unit (RFU) as a function of time.
