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Chunk #7 — Materials and Methods — Image acquisition and preprocessing

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Topological organization of functional brain networks in healthy children: differences in relation to age, sex, and intelligence.
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For each subject, the first ten volumes were discarded to allow for T1 equilibration effects and the adaptation of the subjects to the circumstances, leaving 150 volumes for further analysis. Image preprocessing was carried out using the SPM5 package (http://www.fil.ion.ucl.ac.uk/spm) and Data Processing Assistant for Resting-State fMRI (DPARSF) [28]. First, all functional images were corrected for the acquisition time delay between the slices of each volume using the sinc interpolation and for the geometrical displacement due to head movement using a six-parameter (rigid body) spatial transformation [29]. Nine subjects (6 boys/3 girls) were excluded according to the criteria that head motion was less than 3 mm of displacement or 3 degrees of rotation in any direction. After the correction, the images of 51 subjects (18 boys/33 girls) were normalized to the stereotaxic space [30] using an optimum 12-parameter affine transformation and nonlinear deformations [31] and then resampled to 3-mm isotropic voxels. Finally, the resulting data were further temporally band-pass filtered (0.01–0.1 Hz) to reduce the effects of low-frequency drift and high-frequency physiological noises.