When, first, all 40 variables were forced to enter on the population of 546 subjects (C-group, n = 447; ASD-group, n = 99), group differentiation of C- and ASD-group subjects by Wilks' Lambda (0.278), with Rao's approximation (F = 32.80; df = 40,505; P < 0.0001) was again highly significant. The C-group subjects were classified with 98.7% and the ASD-group patients with 96.0% accuracy.