The TRU subtype associates with gender and smoking habits. However, the expression of ZDHHC5 in LUAD patients of different gender and smoking habits was not significantly different (Supplementary Figures 1C,D). Another character of TRU is the histomorphological feature of acinar, papillary, or lepidic, and these subtypes were associated with a good prognosis. In this study, the clinical pathologist of our group scored ZDHHC5 stained in the IHC samples according to the histomorphology classification (Travis et al., 2015). Unfortunately, due to the relatively small number of LUAD samples in the HPA database (n = 12), we can only confirm that ZDHHC5 can express in the 5 pathological types of Solid, Micropapillary, Acinar, Lepidic, and Enteric, but cannot conclude the prognostic value of ZDHHC5 based on these scores. The above results suggest that although ZDHHC5 gene expression is not associated with gender and smoking, the prognostic value of ZDHHC5 expression in different pathological subtypes should be explored in larger cohorts for future studies.
