We next investigated the ability of PDGFRα+‐OPCs and O4+‐oligodendrocytes to express AMPARs, GABAARs, NMDARs, and GlyRs, each of which have previously been reported to be expressed in oligodendrocyte‐lineage cells 31. Our data indicate that the majority of cells respond to AMPA and GABA and the current density of AMPARsand GABAARs decreases with maturation of PDGFRα+‐OPCs to O4+‐oligodendrocytes. However, no responses to glycine or NMDA were observed in O4+‐oligodendrocytes. Importantly, the maintenance conditions for cultured oligodendrocyte‐lineage cells have been suggested to be a causal factor in reducing the functional expression of both NMDARs and GlyRs 31, 54.
