Dose-response curves for diC8-PIP2/neomycin were fit to the Hill equation (Eq. 3):3\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$y=\frac{{y}_{max}}{1+{(\frac{{x}_{50}}{[x]})}^{h}}$$\end{document}y=ymax1+(x50[x])hwhere ymax is the maximal flux rate or fractional inhibition, [X] is the concentration, h is the Hill coefficient, and x 50 is the half-maximal effective concentration, i.e. EC50 for PIP2 or IC50 neomycin. Dose response curves for modulators, e.g., alcohol, β-OG, capsaicin, do not reach saturation and were normalized to the basal flux rate in the absence of ligand. All values are reported as mean ± S.E.M, with statistical significance (P < 0.05) determined using one-way ANOVA followed by Dunnett’s multiple comparison post hoc test unless otherwise noted. All data were analyzed using Excel (Microsoft) and Prism (GraphPad).
