To build on the differential gene expression signatures observed in the NPC state, we next analyzed the PsychGene NanoString profiles in post-mitotic, neurons that were differentiated for six weeks. As expected, there was an increased expression of the general neural differentiation markers DCX (Doublecortin) and MAP2 (Microtubule-associated protein 2), as well as expression of multiple markers of upper and lower layer cortical projection neurons, including SATB2 (Special AT-rich sequence binding protein 2), CTIP2 (COUP TF-interacting protein 2; BCL11B), ETV1 (Ets variant gene 1), CUX1 (Cut-like homeobox 1), and RELN (Reelin) (Sup. Fig. 11). Comparison of the expression levels in BD-patient neurons relative to their unaffected parental controls revealed that 28 genes in the PsychGene profiles were upregulated (> 1.5 fold change and p-value < 0.05, Benjamini-Hochberg corrected), while 16 genes were down regulated (> 1.5 fold change and p-value < 0.05, Benjamini-Hochberg corrected) (Fig. 4C; Fig. 5B). Given the desire to gain insight into specific neuronal subtypes that may underlie alterations in the neurocircuitry affected in BD, analysis of the expression of markers of specific neuron subtypes revealed differential expression
