Five reporter assay plasmid constructs were generated containing the region upstream of the CHRNB3 gene, shown in Figure 2. All of the known SNPs (identified from the Ensembl and UCSC Genome Browsers) are shown, approximately to scale. The top three long inserts consisted of approximately 3000 bp which includes the putative promoter region of CHRNB3. Three different long plasmid constructs, as indicated by the genotype for each SNP, were cloned representing common (B3L_common), mixed (B3L_mixed) and minor (B3L_minor) haplotypes. The bottom pair containing shorter inserts of ~1500 bp represents two haplotypes (B3S_common and B3S_minor).
