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Chunk #11 — Materials and Methods — Operant Training

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Muscarinic type 2 receptors in the lateral dorsal tegmental area modulate cocaine and food seeking behavior in rats.
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Operant conditioning chambers (Med Associates Inc.; St. Albans, VT) were housed inside sound attenuating boxes and were equipped with a retractable active lever, an inactive lever, two 4W stimulus lights positioned 2cm above each lever, a 10W house light, a pellet dispenser and a pellet receptacle tray located between the two levers (Hansen and Mark, 2007). A syringe pump for cocaine delivery was located outside of the sound attenuating box. Presses of the active lever resulted in delivery of a reward (either a food pellet or cocaine infusion) and retraction of the lever. Pressing the inactive lever had no scheduled consequence.