To investigate whether neuronal subpopulations are similarly affected in Pcdhgtcko/tcko and Pcdhgdel/del mutants, we examined several classes of interneurons in the ventral spinal cord at P0. Interestingly, while Pax2+ and Foxp2+ inhibitory interneurons, as well as Chx10+ excitatory interneurons are similarly reduced in number in both mutants, V1-derived Calbindin (CB)+ Renshaw cells and V0-derived cholinergic ChAT+ partition cells are spared (Figures 2C–G, 2I). In conclusion, the Pcdhgtcko/tcko and Pcdhgdel/del mutants display similar levels and patterns of neuronal cell loss in the spinal cord, and interneuron subpopulations are differentially affected in both mutants.
