neurites towards a predetermined direction. In addition, this approach has not been utilized in iPSC-derived neurons [52]. We found that neurons plated on star-shaped micropatterned substrates initially formed individual connection to neighboring cell bodies either by the axon or the dendrites and were able to form a more dense and organized network over time. Micropatterned substrates provide an excellent environment to study the development of neuronal polarity in human neurons, because they allow for the distinction of axons and dendrites on the surface due to their separation on cell-adhesive areas.
