was observed in Ts65Dn:Kcnj6+++ vs. Ts65Dn:Kcnj6++− mice (F1,13 = 2.15; p = 0.025), while there was no difference between Ts65Dn:Kcnj6++− and 2N:Kcnj6++ groups (F1,35 = 0.11; p = 0.46) (Fig. 5B). One factor affecting long-term memory is anxiety. To assess levels of anxiety during the test, we counted numbers of pellets dropped by mice. There was no difference between the groups (p = 0.60–0.93) indicating that the levels of anxiety were equal among all groups. Thus, restoration of Kcnj6 gene dose led to restoration of long-term memory in Ts65Dn mice.
