We also quantified the relationship between p120 and E-cadherin expression by FACS® analysis of a population of p120 siRNA-infected cells with mAb-HECD1, which recognizes the extracellular domain of human E-cadherin (Fig. 3 b). The cells were sorted into pools with progressively decreasing levels of surface E-cadherin. Cell lysates were generated from each pool, divided in half, and then Western blotted for E-cadherin and p120 (Fig. 3 b). As in the immunofluorescent assays, the levels of p120 closely paralleled the levels of E-cadherin.
