The list of tissue-specific genes was obtained from (Mele et al., 2015). Transcriptome profiles of all available brain regions in 8 and 9 pcw were downloaded from BrainSpan database (http://www.brainspan.org/). Genes with more than 2 fold change and T test p<0.05 were used as gene signatures of each in vivo brain region. Enrichment of these gene sets were evaluated by Gene Set Enrichment Analysis (GSEA) software (v2.2.2) with 1,000 permutations of gene sets, weighted enrichment statistic and signal-to-noise separation metric (Figure 4A and 4D).
