Chunk #48 — STAR Methods — Experimental Model and Subject Details — Cell Culture — Differentiation of iHPCs to iMGLs

Source

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.

Embedded

yes

Text

iMGL Differentiation Base Medium: Differentiation media consists a base media: phenol-free DMEM/F12 (1:1), insulin (0.2 mg/ml), holo-transferrin (0.011 mg/ml), sodium selenite (13.4 μg/ml) (can use ITS-G, 2%v/v,Thermo Fisher Scientific), B27 (2% v/v), N2 (0.5%, v/v), monothioglycerol (200 μM), Glutamax (1X), NEAA (1X), and additional insulin (5 μg/ml; Sigma). Use 0.22 μm filter.