Human iPS cell lines were generated by RUCDR Infinite Biologics ® from human primary lymphocytes carrying either MOR N40 or D40 genotypes using Sendai viral vectors (CytoTune™, ThermoFisher Scientific), as previously described 23. Human iPS cells were cultured and maintained as described previously 24. Human iPS cells were cultured in 37°C, 5% CO2 on Matrigel® Matrix (Corning Life Sciences)- coated plates in mTeSR medium (Stem Cell Technologies). For passaging and differentiation, done weekly, iPS cells were dissociated using Accutase (Stem Cell Technologies), spun down at 1000 rpm for 5 minutes, and re-plated at a density of 20,000 cells/cm2 for maintenance cultures and 50,000 cells/cm2 for differentiation.
