In order to rapidly develop a broad base of behavioral phenotyping data, phenotyping was performed in several multi-variate test batteries (Table 1). Each battery consists of a set of tests administered serially to individual mice. This approach also allows for the examination of partial-correlation within strain (non-genetic correlation) but may upwardly bias estimates of genetic correlation obtained using strain means of the measures. A given mouse was assigned to one and only one battery and received all tests in that battery. The order of repeated testing (reported in Table 1) was either fixed where logical and necessary or, in the case of the nociception battery, varied systematically using randomly generated Latin-square designs each applied to a different strain and sex. In general, for fixed-order batteries, the least stressful measures were obtained first, and all baseline measures were necessarily obtained before conditioning or drug exposures. The testing protocols were largely derivative of those developed in consultation with the external advisory board of the Tennessee Mouse Genome Consortium (TMGC) ENU-Neuromutagenesis Program (Goldowitz et al. 2004).
