Chunk #18 — Materials and methods — Differentiation of hESCs into cortical interneurons and analysis of ethanol-induced miRNA and mRNA transcriptomic changes by RNA-seq
miRNA transcriptomes of hESC-derived neurons (exposed or unexposed to ethanol) were profiled by small RNA-seq and the raw data obtained from small RNA-seq was processed by CAP-miRseq [29], as described above. The mean total number of reads per sample was 29,145,212, and the mean mapping rate (aligned reads/reads sent to Aligner) was 85.0%. The quality of the miRNA-seq data was visualized using box plots (Fig. S5a). The miRNA-seq data has been deposited in the NCBI GEO database (accession number: GSE181050).
