Interactions between ethanol and PKC have been more extensively investigated than PKC interactions with other addictive drugs. Similar to stimulants and opioids, ethanol alters the localization and abundance of PKC. Acute ethanol treatment induces PKC translocation in cultured astroglial cells.185 In the neuroblastoma × glioma hybrid cell line NG108-15, chronic ethanol exposure also induces translocation of PKCδ and PKCε;186 this translocation of PKCε is to the cytosol in a complex with εRACK and is mediated by PKA, possibly through PKA phosphorylation of εRACK.187 In vivo ethanol administered i.p. stimulates phosphorylation of cerebellar PKCε at Ser-729, and this increase in phosphorylated PKCε correlates with the development of acute functional tolerance to the motor-impairing effects of ethanol in mice that is PKCε-dependent.188
