Mitochondria not only show dynamic movement within the mitochondrial network, but also show motility throughout neurons [29]. The trafficking of mitochondria to areas of higher energy requirements, such as synapses, where mitochondrial densities fluctuate, further highlights the importance of efficient mitochondrial dynamics in neurons. Specific trafficking machineries enable mitochondrial transport to synaptic areas of increased energy demand or to the cell body for degradation [30]. Mitochondria move along microtubules using motor and adaptor proteins. Anterograde movement (towards the axon terminal) is dependent on kinesin motors, while retrograde movement utilizes dynein motors [31]. Impaired mitochondrial transport has been shown previously to be implicated in numerous age-related neurodegenerative disorders [32]. A number of model systems have been established to study mitochondrial movement in neurons [33-35]. We used an insect cell virus (baculovirus) coupled with a mammalian promotor to label cellular organelles. We transduced iPSC-derived neurons 16 hours prior to imaging with the BacMam CellLight® Mitochondria-GFP construct, which utilizes the leader sequence of E1 alpha-pyruvate dehydrogenase (3.1 kDa) to label mitochondria. By adjusting the relative number of viral particles, it was possible to
