Coronal slices containing the VTA (200-300 μm) and sagittal slices containing the NAcc (240 μm) were prepared from drug-naïve wild-type and Girk−/− mice (4-6 weeks), and allowed to recover in oxygenated ACSF at room temperature for >1 h. Slices were transferred to recording chambers and perfused with oxygenated ACSF (+/− drugs) at a flow rate of approximately 2-2.5 mL/min during experiments. Currents, resistances, and potentials were measured using Multiclamp 700A amplifiers and pCLAMP v.9 software (Molecular Devices; Foster City CA) and stored on hard disk. All measured and command potentials factored in junction potentials predicted using JPCalc software (Molecular Devices).
