We performed quality control on the genome-edited cell lines. PGP1e-TAZc.517delG cells had a normal karyotype (Suppl. Fig. 3a), expressed the pluripotency genes OCT4 and NANOG at levels comparable to the human ES cell line H7 (Suppl. Fig. 3b–c), and differentiated into all three germ layers in teratoma assays (Suppl. Fig. 3d–g). The cell lines differentiated efficiently into cardiomyocytes using a common directed differentiation protocol (Suppl. Fig. 3h).16 Indeed, we showed that the genome-edited PGP1e-TAZc.517delG iPSC line effectively recapitulates hallmarks of Barth syndrome (Suppl. Fig. 4).
