Chunk #40 — Methods — Quantitative assessment of D1, D2 and D1/D2 MSNs in the striatum

Source

Striatal Neurons Expressing D and D Receptors are Morphologically Distinct and Differently Affected by Dopamine Denervation in Mice.

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In the 4 sham-lesioned animals, the 2 transverse sections taken at the pre-commissural (0.26 mm from bregma) and post-commissural (−0.94 mm from bregma) striatal levels that were immunostained for MOR were used to estimate the number of D1/D2 MSNs in the striosomes and matrix compartments. Again, all striatal D1/D2 MSNs were counted from previously acquired confocal images and the striosomes and matrix compartments were delineated by using MOR immunoreactivity.