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Chunk #15 — Methods — Identifying differentially expressed genes (DEGs)

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Integrative transcriptome network analysis of iPSC-derived neurons from schizophrenia and schizoaffective disorder patients with 22q11.2 deletion.
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Although we have biological replicates for a few iPSC lines, we found that intra-individual variations were as large as inter-individual variations. The correlation of coefficients (CV) for two controls with duplications were 0.25 and 0.28 and for two SZ with duplications were 0.17 and 0.34. These numbers were very similar to the CVs observed for inter-individuals, 0.26 for controls and 0.28 for SZ samples. As such, we analyzed all samples together without specifically weighting or nested analysis of the samples derived from the same individuals.