Forward electronic PCR (e-PCR) searches for matches to Sequence Tagged Site (STS) primer pairs in the UniSTS database of over 520 000 markers. Reverse e-PCR is used to estimate the genomic binding site, amplicon size and specificity for sets of primer pairs by searching against genomic and transcript databases. Both e-PCR binaries and source code are available at ftp.ncbi.nlm.nih.gov/pub/schuler/e-PCR.
