Lipodystrophy mice were generated at Amgen Inc. by cloning the mature human SREBP1c (residues 1–436) downstream of the mouse adipocyte fatty acid binding protein aP2 promoter. Briefly, a 1450 b.p. cDNA encompassing human SREBP1c was cloned into a transgenic vector containing the mouse 5.4 Kb aP2 promoter/enhancer genomic fragment and the SV40 small T intron and polyadenylation signal. The transgene was excised with Sph2 and Swa1 and purified for microinjection into FVB zygotes. Following confirmation of transgene overexpression by northern blot (Figure S1A), transgenic mice (Tg) were housed and bred at Charles River Laboratories (San Diego, CA), and maintained by continued backcrossing of Tg males to FVB females.
