In the human embryonic stem cell (hESC) studies, line H9 (WA09) (WiCell Research Institute, Inc.) and line HUES8 (originated from Harvard University, Boston, USA) were used. The iPSC lines used in the present study (Table 1) were derived from different control individuals and from individuals diagnosed with schizophrenia at different ages33. In earlier studies these schizophrenia lines displayed similar phenotypic changes in 2D cultures as described in the section “Introduction”31,33,42. Recent RNAseq investigation showed common dysregulation of a global transcriptome in neural progenitor cells (NPCs) differentiated from all schizophrenia lines and ChIPseq showed similar changes in nFGFR1 genomic interactions in two investigated schizophrenia iPSC lines29. In the present study, we found no discernable differences between the organoids generated from control female and control male iPSC lines and between schizophrenia female and control male iPSC lines. The differences between schizophrenia and control organoids were reproduced in all control and schizophrenia iPSC lines and were statistically evaluated.Table 1iPSC lines used to generate organoids29,33 Cell line IDGenderDescriptionAge of diagnosis2038MaleiPSC SCZ6 years2497MaleiPSC SCZ15 years1835FemaleiPSC SCZ27 yearsBJ #1MaleiPSC Control3440MaleiPSC Control2937MaleiPSC Control3651FemaleiPSC Control
