RNA samples were denatured, resolved by formaldehyde agarose electrophoresis and blotted to Gene Screen (Dupont) by high salt capillary transfer according to standard procedures. Blots were probed with random hexamer labeled DNA probes using Prefect Hyb hybridization solution (Sigma) at 65°C according to the manufactures procedures. Probes used were the Probe B and Exon2 probe described above. Blots were washed with four times with 1× SSC for 10 minutes at 65°C. Phosphoimager was used to detect the hybridization signal.
