cells the majority were PV positive (88.5%, 22.5%, and 64.4% in layer II/III, layer IV/V, and layer VI, respectively in Figure 5B). Interestingly, PGC-1α mRNA signals were dramatically reduced following PWSI in the cortex of KO mice (Figure 5C). Quantitative RT-PCR using mPFC total RNA confirmed that PWSI elicits down-regulation of PGC-1α expression only in KO mice at 8 weeks old (Figure 5D). Reduced PGC-1α in KO mice following PWSI was also detectable at the protein level, as assessed by Western blots (Figure 5E). Interestingly, while PGC-1α mRNA levels were reduced at 16 weeks old regardless of housing conditions, APO treatment encompassing the entire PWSI period in KO mice alleviated the reduction (Figure 5F). As over 80% of cortical PV-positive cells in the KO mice are cre-targeted (15) and most of PV cells are PGC-1α positive, this suggested that the down regulation of PGC-1α in the KO mice are mostly in the GluN1-deleted neurons. While 24% of cre-targeted cells are Reelin positive, there was no PGC-1α expression in these Reelin-positive cre cells (Figure S4 in Supplement 1).
